Table 7.

Cost analysis for DNA extraction procedure F (according to Singka et al., 2012).

Consumables	Volumes	Units	Concentration	Volumes	/Weight	High costs			Low costs			Low cost	High cost
						Amount	Unit	Fix cost (€)	Amount	Unit	Fix cost (€)	per Prep (€)	per Prep (€)
Gloves (any size)	1	pair	–	–	–	50	pair	8.20	50	pair	4.50	0,.900	0.1640
Tubes	4	–	–	1.5	mL	500	pieces	8.20	1000	pieces	14.90	0.0596	0.0656
Tips	12		–	1000	µl	500	pieces	5.08	1000	pieces	7.70	0.0924	0.1218
Tips	2		–	200	µL	500	pieces	5.40	1000	pieces	8.19	0.0164	0.0216
Glass beads 0.1 mm	0.5	g	–	5.0	g	1000	g	24.30	25000	g	202.00	0.0404	0.1215
CTAB extraction buffer	0.5	mL	0.7 M NaCl	10.2	mg	500	g	15.84	1000	g	24.19	0.0002	0.0003
(1:1 v/v) 10% w/v (CTAB in NaCl)			10% w/v CTAB	2,5	µg	100	g	22.64	1000	g	89.11	2.2E-07	5.7E-07
to KH2PO4			240 mM KH2PO4	8.2	mg	250	g	19.66	1000	g	56.66	0.0005	0.0006
Phenol:Chloroform:Isoamyl	1.0	mL	25′ Phenol	0.5	g	100	g	18.00	1000	g	64.40	0.0322	0.0900
(25:24:1 v/v)			24′ Chloroform	0.48	mL	500	mL	50.62	2500	mL	100.66	0.0193	0.0486
			1′ Isoamyl	0.02	mL	25	mL	13.92	1000	mL	108.00	0.0022	0.0111
Chloroform:Isoamyl	0.5	mL	24' Chloroform	0.48	mL	500	mL	50.62	2500	mL	100.66	0.0193	0.0486
(24:1 v/v)			1′ Isoamyl	0.02	mL	25	mL	13.92	1000	mL	108.00	0.0022	0.0111
Sodium acetate (0.1:1 v/v)	0.05	mL	3 M	12.3	mg	250	g	22.47	1000	g	56.30	0.0007	0.0011
Isopropanol (0.6: 1 v/v)	0.3	mL	100%	0.3	mL	1000	mL	30.30	2500	mL	61.70	0.0074	0.0091
Ethanol	1.5	mL	70%	1.05	mL	250	mL	47.56	2500	mL	246.58	0.1036	0.1998
TE buffer	0.1	mL	10 mM Tris–HCl	0.12	mg	500	g	93.40	1000	g	128.00	1.6E-05	2.3E-05
			1 mM EDTA	0.03	mg	100	g	34.08	1000	g	245.23	7.2E-06	1.0E-05
Extracted samples	12					–			–			–	–
Extraction time	195	min				–		–	–		–	–	–
Lab staff (per hour)						–		35.00	–		25.00	–	–
Lab staff (€/extraction)												6.77	9.48
Chemicals (€/extraction)												0.19	0.42
Gloves, tubes, tips (€/extraction)												0.30	0.49
Final price per extraction including extraction time, lab staff and all consumables (€)												7.26	10.39