Table 9.

Cost analysis for DNA extraction procedure H (according to Tabatabaei et al. [5]).

Consumables	Volumes	Units	Concentration	Volumes	/Weight	High costs			Low costs			Low cost	High cost
						Amount	Unit	Fix cost (€)	Amount	Unit	Fix cost (€)	per Prep (€)	per Prep (€)
Gloves (any size)	1	pair	–	–	–	50	pair	8.20	50	pair	4.50	0.0900	0.1640
Tubes	3	–	–	2.0	mL	500	pieces	11.90	1000	pieces	21.90	0.0657	0.0714
Tips	12		–	1000	µL	500	pieces	5.08	1000	pieces	7.70	0.0924	0.1218
Tips	1		–	200	µL	500	pieces	5.40	1000	pieces	8.19	0.0082	0.0108
Tips	0		–	10	µL	1000	pieces	27.14	2000	pieces	43.42	0.0000	0.0000
EDTA	0.4	mL	0.5 EDTA	58.4	mg	100	g	34.08	1000	g	245.23	0.0143	0.0199
Lysis buffer	0.4	mL	10 mM Tris	0.48	mg	500	g	93.40	1000	g	128.00	0.0001	0.0001
			1 mM EDTA	0.12	mg	100	g	34.08	1000	g	245.23	3.E-05	4.E-05
			2 mg mL-1 Lysozyme	0.80	mg	1,0	g	23.89	10	g	96.04	0.0077	0.0191
SDS	0.05	mL	10% w/v	0.005	mg	100	g	16.56	1000	g	56.48	2.8E-07	8.3E-07
Phenol:Chloroform	0.8	mL	Phenol	0.4	g	100	g	18.00	1000	g	64.40	0.0258	0.0720
(1:1 v/v)			Chloroform	0.4	mL	500	mL	50.62	2500	mL	100.66	0.0161	0.0405
Sodium acetate	0.08	mL	3 M	19.7	mg	250	g	22.47	1000	g	56.30	0.0011	0.0018
Isopropanol	0.9	mL	100%	0.9	mL	1000	mL	30.30	2500	mL	61.70	0.0222	0.0273
Ethanol	1.5	mL	70%	1.05	mL	250	mL	47.56	2500	mL	246.58	0.1036	0.1998
TE buffer	0.1	mL	10 mM Tris–HCl	0.12	mg	100	g	34.08	1000	g	245.23	3.0E-05	4.1E-05
			1.0 mM EDTA	0.03	mg	500	g	93.40	1000	g	128.00	3.7E-06	5.5E-06
Extracted samples	12					–			–		–	–	–
Extraction time	210	min				–		–	–		–	–	–
Lab staff (per hour)						–		35.00	–		25,00	–	–
Lab staff (€/extraction)												7.29	10.21
Chemicals (€/extraction)												0.19	0.38
Gloves, tubes, tips (€/extraction)												0.26	0.37
Final price per extraction (€)												7.74	10.96