Table 10.
Cost analysis for DNA extraction procedure I (according to Tresse et al. [6]).
| Consumables | Volumes | Units | Concentration | Volumes | /Weight |
High costs |
Low costs |
Low cost | High cost | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Amount | Unit | Fix cost (€) | Amount | Unit | Fix cost (€) | per Prep (€) | per Prep (€) | ||||||
| Gloves (any size) | 1 | pair | – | – | – | 50 | pair | 8.20 | 50 | pair | 4.50 | 0.0900 | 0.1640 |
| Tubes | 3 | – | – | 2.0 | mL | 500 | pieces | 11.90 | 1000 | pieces | 21.90 | 0.0657 | 0.0714 |
| 4 | – | – | 1.5 | mL | 500 | pieces | 8.20 | 1000 | pieces | 14.90 | 0.0596 | 0.0656 | |
| Tips | 14 | – | 1000 | µL | 500 | pieces | 5.08 | 1000 | pieces | 7.70 | 0.1078 | 0.1421 | |
| Tips | 4 | – | 200 | µL | 500 | pieces | 5.40 | 1000 | pieces | 8.19 | 0.0328 | 0.0432 | |
| Tips | 1 | – | 10 | µL | 1000 | pieces | 27.14 | 2000 | pieces | 43.42 | 0.0217 | 0.0271 | |
| TEN buffer | 0.7 | mL | 100 mM Tris | 8.48 | mg | 500 | g | 93.40 | 1000 | g | 128.00 | 0.0011 | 0.0016 |
| 100 mM EDTA | 20.45 | mg | 100 | g | 34.08 | 1000 | g | 245.23 | 0.0050 | 0.0070 | |||
| 100 mM NaCl | 4.09 | mg | 500 | g | 15.84 | 1000 | g | 24.19 | 9.9E-05 | 1.3E-04 | |||
| 5 mg mL-1 Lysozyme | 3.5 | mg | 1.0 | g | 23.89 | 10 | g | 96.04 | 0.0336 | 0.0836 | |||
| SDS | 0.035 | mL | 20% w/v | 0.007 | mg | 100 | g | 16.56 | 1000 | g | 56.48 | 4.0E-07 | 1.2E-06 |
| Proteinase K | 0.01 | mL | 20 mg mL-1 | 0.2 | mg | 100 | mg | 67.68 | 500 | mg | 259.62 | 0.1038 | 0.1354 |
| Silica beads | – | ID 0.1 mm | 250 | mg | 1000 | g | 24.30 | 25000 | g | 202.00 | 2.0E-03 | 0.0061 | |
| Silica beads | – | ID 0.5 mm | 250 | mg | 1000 | g | 25.23 | 20000 | g | 227.18 | 0.0028 | 0.0063 | |
| Silica beads | 2 | beads | ID 6.0 mm | 69 | mg | 500 | g | 34.20 | 1000 | g | 12.35 | 0.0009 | 0.0047 |
| Ammoniumacetate | 0.145 | mL | 10 M | 111.8 | mg | 250 | g | 15.30 | 1000 | g | 45.29 | 0.0051 | 0.0068 |
| RNase A | 0.005 | mL | 1 mg mL-1 | 0.005 | mg | 250 | mg | 94.40 | 1000 | mg | 292.00 | 0.0015 | 0.0019 |
| Phenol:Chloroform:Isoamyl | 1.5 | mL | 25' Phenol | 0.75 | g | 100 | g | 18.00 | 1000 | g | 64.40 | 0.0483 | 0.1350 |
| (25:24:1 v/v) | 24' Chloroform | 0.72 | mL | 500 | mL | 50.62 | 2500 | mL | 100.66 | 0.0290 | 0.0729 | ||
| 1' Isoamyl | 0.03 | mL | 25 | mL | 13.92 | 1000 | mL | 108.00 | 0.0032 | 0.0167 | |||
| Chloroform:Isoamyl | 0.7 | mL | 24' Chloroform | 0.672 | mL | 500 | mL | 50.62 | 2500 | mL | 100.66 | 0.0271 | 0.0680 |
| (24:1 v/v) | 1' Isoamyl | 0.028 | mL | 25 | mL | 13.92 | 1000 | mL | 108.00 | 0.0030 | 0.0156 | ||
| Sodiumaceate (1:10 v/v) | 0.07 | mL | 3 M | 17.2 | mg | 250 | g | 22.47 | 1000 | g | 56.30 | 0.0010 | 0.0015 |
| Ethanol (2:1 v/v) | 1.4 | mL | 98% | 1.37 | mL | 250 | mL | 47.56 | 2500 | mL | 246.58 | 0.1353 | 0.2610 |
| Ethanol | 1.0 | mL | 70% | 0.7 | mL | 250 | mL | 47.56 | 2500 | mL | 246.58 | 0.0690 | 0.1332 |
| TE buffer | 0.1 | mL | 10 mM Tris–HCl | 0.12 | mg | 100 | g | 34.08 | 1000 | g | 245.23 | 3.0E-05 | 4.1E-05 |
| 1.0 mM EDTA | 0.03 | mg | 500 | g | 93.40 | 1000 | g | 128.00 | 3.7E-06 | 5.5E-06 | |||
| Extracted samples | 12 | – | – | – | – | – | |||||||
| Extraction time | 170 | min | – | – | – | – | – | – | |||||
| Lab staff (per hour) | – | 35.00 | – | 25.00 | – | – | |||||||
| Lab staff (€/extraction) | 5.90 | 8.26 | |||||||||||
| Chemicals (€/extraction) | 0.47 | 0.96 | |||||||||||
| Gloves, tubes, tips (€/extraction) | 0.38 | 0.51 | |||||||||||
| Final price per extraction including extraction time, lab staff and all consumables (€) | 6.75 | 9.73 | |||||||||||