Table 11.
Cost analysis for DNA extraction procedure J (according to Wilson [7]).
| Consumables | Volumes | Units | Concentration | Volumes | /Weight |
High costs |
Low costs |
Low cost | High cost | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Amount | Unit | Fix cost (€) | Amount | Unit | Fix cost (€) | per Prep (€) | per Prep (€) | ||||||
| Gloves (any size) | 1 | pair | – | – | – | 50 | pair | 8.20 | 50 | pair | 4.50 | 0.0900 | 0.1640 |
| Tubes | 3 | – | – | 2.0 | mL | 500 | pieces | 11.90 | 1000 | pieces | 21.90 | 0.0657 | 0.0714 |
| Tips | 9 | – | 1000 | µL | 500 | pieces | 5.08 | 1000 | pieces | 7.70 | 0.0693 | 0.0914 | |
| Tips | 4 | – | 200 | µL | 500 | pieces | 5.40 | 1000 | pieces | 8.19 | 0.0328 | 0.0432 | |
| Tips | 1 | – | 10 | µL | 1000 | pieces | 27.14 | 2000 | pieces | 43.42 | 0.0217 | 0.0271 | |
| TE buffer | 0.567 | mL | 10 mM Tris | 0.69 | mg | 100 | g | 34.08 | 1000 | g | 245.23 | 0.0002 | 0.0002 |
| 10 mM EDTA | 1.66 | mg | 500 | g | 93.40 | 1000 | g | 128.00 | 0,0002 | 0.0003 | |||
| SDS | 0.03 | mL | 10% w/v | 0.003 | mg | 100 | g | 16.56 | 1000 | g | 56.48 | 1.7E-07 | 5.0E-07 |
| Proteinase K | 0.003 | mL | 20 mg mL-1 | 0.06 | mg | 100 | mg | 67.68 | 500 | mg | 259.62 | 0.0312 | 0.0406 |
| NaCl | 0.1 | mL | 5 M | 29.22 | mg | 500 | g | 15.84 | 1000 | g | 24.19 | 7.1E-04 | 9.3E-04 |
| CTAB/NaCl | 0.08 | mL | 0,7 M NaCl | 3,3 | mg | 500 | g | 15.84 | 1000 | g | 24.19 | 0.0001 | 0.1037 |
| 10% w/v CTAB | 0.008 | mg | 100 | g | 22.64 | 1000 | g | 89.11 | 7.1E-07 | 1.8E-06 | |||
| Chloroform:Isoamyl | 1.0 | mL | 24' Chloroform | 0.96 | mL | 500 | mL | 50.62 | 2500 | mL | 100.66 | 0.0387 | 0.0972 |
| (24:1 v/v) | 1' Isoamyl | 0.04 | mL | 25 | mL | 13.92 | 1000 | mL | 108.00 | 0.0043 | 0.0223 | ||
| Phenol:Chloroform:Isoamyl | 0.9 | mL | 25' Phenol | 0.45 | g | 100 | g | 18.00 | 1000 | g | 64.40 | 0.0290 | 0.0810 |
| (25:24:1 v/v) | 24' Chloroform | 0.432 | mL | 500 | mL | 50.62 | 2500 | mL | 100.66 | 0.0174 | 0.0437 | ||
| 1' Isoamyl | 0.018 | mL | 25 | mL | 13.92 | 1000 | mL | 108.00 | 0.0019 | 0.0100 | |||
| Isopropanol (0.6: 1 v/v) | 0.3 | mL | 100% | 0.3 | mL | 1000 | mL | 30.30 | 2500 | mL | 61.70 | 0.0074 | 0.0091 |
| Ethanol | 0.5 | mL | 70% | 0.35 | mL | 250 | mL | 47.56 | 2500 | mL | 246.58 | 0.0345 | 0.0666 |
| TE buffer | 0.1 | mL | 10 mM Tris–HCl | 0.12 | mg | 100 | g | 34.08 | 1000 | g | 245.23 | 3.0E-05 | 4.1E-05 |
| 1.0 mM EDTA | 0.03 | mg | 500 | g | 93.40 | 1000 | g | 128.00 | 3.7E-06 | 5.5E-06 | |||
| Extracted samples | 12 | – | – | – | – | – | – | ||||||
| Extraction time | 105 | min | – | – | – | – | – | – | |||||
| Lab staff (per hour) | – | 35.00 | – | 25.00 | – | – | |||||||
| Lab staff (€/extraction) | 3.65 | 5.10 | |||||||||||
| Chemicals (€/extraction) | 0.17 | 0.48 | |||||||||||
| Gloves, tubes, tips (€/extraction) | 0.28 | 0.40 | |||||||||||
| Final price per extraction including extraction time, lab staff and all consumables (€) | 4.09 | 5.98 | |||||||||||