Table 2. Generation of chimeric rats by blastocyst injection with ahES-2 and phES-2 cells.
Cell source * | Blastocysts transferred | E14.5 fetuses + full-term offspring (%) | Chimeric fetuses + offspring (%) |
ahES-2 haploid | 17 + 65 | 10 + 41 (62) | 0 + 0 (0) |
ahES-2 diploid | 35 + 16 | 30 + 10 (78) | 11 + 5 (40) |
phES-2 haploid | 20 + 62 | 11 + 47 (71) | 0 + 1 (2) |
phES-2 diploid | 26 + 16 | 12 + 15 (64) | 0 + 0 (0) |
* Cells from the ahES-2 and phES-2 cell lines were FACS-purified prior to blastocyst injection.