Table 1. Sequence of primers, annealing temperature (Ta), amplicon size and number od CpG sites present.
S.No. | Primer Sequences | Ta | Amplicon length | CpG sites |
---|---|---|---|---|
1 | F: 5′TTTTAATTTTTGTTTGGAGGGTAGT-3′ R: 5′AAAAAAACCACTTATCACCAAATTC-3′ |
55°C | 155bp | 7 |