Fig. 2.

Endothelial cells and macrophages are SASP-carrying cells in the diabetic mice kidney. A. Representative confocal microscope images of kidney sections stained for p16 (red) from control (Ctrl) and hyperglycaemic mice (HG-M). Nuclei are counterstained with DAPI (blue). Scale bar = 50 µm. B. Experimental design used to isolate macrophages, ECs, and non-macrophagic/non-endothelial cells from kidneys. C. Fold changes in sorted populations in expression levels of SA and SASP mRNAs (p16, p21, IL-6, TGF-β, PAI-1 and MCP-1) (n=3 mice/group). Blue histograms = means of vehicle-treated mice (Ctrl); red histograms = means of STZ-treated hyperglycaemic mice (HG-M). For all diagrams error bars are ± SD and * t-test p < 0.05, ** t-test p < 0.01 (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.).