Table 1.
Primer sequence, fragment size, and annealing temperature.
| Gene name | Forward | Backward | Fragment size (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
| hGAPDH (NM_001289746.1) | CACTAGGCGCTCACTGTTCTCT | CGTTCTCAGCCTTGACGGT | 250 | 66 |
| hDSPP (NM_014208.3) | TGCTGGCCTGGATAATTCCG | CTCCTGGCCCTTGCTGTTAT | 136 | 66 |
| hBSP (NM_004967.3) | AAGGGCACCTCGAAGACAAC | CCCTCGTATTCAACGGTGGT | 119 | 62.8 |
| hITGA1 (NM_181501.1) | CTCACTGTTGTTCTACGCTGC | ACGACTTGAAATGTGGGGCT | 419 | 59.9 |
| hITGA2 (NM_002203.3) | GTGGCTTTCCTGAGAACCGA | GAAGCTGGCTGAGAGCTGAA | 278 | 62.8 |
| hITGA3 (NM_002204.3) | ATGGCAAGTGGCTGCTGTAT | GCACTCTAGCCACACACAGT | 272 | 59.9 |
| hITGA4 (NM_000885.5) | AATCCCGGGGCGATTTACAG | TCCAGCTTGACATGATGCAAAA | 354 | 59.9 |
| hITGA5 (NM_002205.4) | CCCTCATCTCCGGGACACTA | ATCCAACTCCAGGCCCTTTG | 397 | 56.3 |
| hITGA6 (NM_000210.3) | CTCGCTGGGATCTTGATGCT | TCAGATGGCTGAGCATGGAT | 128 | 59.9 |
| hITGA7 (NM_002206.2) | GGAAGACCGACAGCAGTTCA | ATCTTGATGCGACACCAGCA | 264 | 59.9 |
| hITGA8 (NM_003638.2) | GCCTATGCCGAGTTCTCTCC | CCCAGTAAACTCCCCAGCAG | 297 | 59.9 |
| hITGB1 (NM_002211.3) | GCCGCGCGGAAAAGATGAAT | TGCTGTTCCTTTGCTACGGT | 323 | 59.9 |
| hITGB3 (NM_000212.2) | GAAGCAGAGTGTGTCACGGA | ACATGACACTGCCCGTCATT | 201 | 59.9 |