Figure 3. Overexpression of miR-503 suppressed the functions of pancreatic β cell. miR-503 mimics was transfected into INS-1 cells pretreated with 5.5 mM glucose or 22 mM high glucose for 24 h. A. Insulin mRNA levels were measured by qRT-PCR. B. Total insulin content was measured by ELISA assay. C. Cell viability was determined by MTT assay. D. Cell apoptosis was detected by flow cytometry. Data represent the mean ± SD of three independent experiments. **p < 0.01 compared with mimics NC or Blank group.
