Pathogenicity and transmission of A(H3N2) canine influenza virus in ferrets. Six ferrets were intranasally inoculated with 107.1 50% egg infective doses (EID50) of A/canine/IL/12191/2015 virus. A, The following day, a serologically naive ferret was placed in the same cage with an inoculated ferret for the assessment of virus transmission between 3 ferret pairs in direct contact. Nasal wash titers from individual ferrets are presented. Viral titers are presented as log10 EID50/mL. B, Three inoculated ferrets were euthanized 3 days after inoculation, and tissues were collected for assessment of viral titers. Blood and nasal turbinate viral titers are presented as log10 EID50/mL, and kidney, spleen, liver, intestines (pooled duodenum, jejunoileal loop, and descending colon), olfactory bulb, brain (pooled anterior and posterior brain), lung, and trachea are presented as log10 EID50/g of tissue. The limit of detection is 1.5 log10 EID50 per g or mL.