Table 3.

PCR primers used in this study

Primer	Sequence (5′–3′)
3′ mCherry	agatctgtcgacgcggccgcTTACTTGTACAGCTCGTCCATGCCG
3′ mCherryHDELR	agatctgtcgacgcggccgcTTAGCGTAGCTCATCGTGCTTGTACAGCTCGTCCATGCCGC
3′ mTFPHDELR	agatctgtcgacgcggccgcTTAGCGTAGCTCATCGTGCTTGTACAGCTCGTCCATGCCGTC
3′ SOE β-mCherry	CCTCCTCGCCCTTGCTCACCATGCACCTTGCCTCGTCGTCACC
3′ SOE β-mTFP	GTCTCCTCGCCCTTCGTCACCATGCACCTTGCCTCGTCGTCACC
5′ K28 wo SP	agatctctcgagAAAAGAATGCCGACATCTGAGAGACAGCAGGG
5′ mCherry	agatctctcgcgAAAAGAATGCCGACATCTGAGAGACAGCAGGG
5′ mTFP	agatctctcgagAAAAGAATGGTGAGCAAGGGCGAGGAGAC
5′ SOE β-mCherry	GGTGACGACGAGGCAAGGTGCATGGTGAGCAAGGGCGAGGAGG
5′ SOE β-mTFP	GGTGACGACGAGGCAAGGTGCATGGTGAGCAAGGGCGAGGAGAC

Restriction endonuclease cleavage sites used for cloning are shown in small form letters