Fig. 3.

Phenotype of autologous DRibbles derived from pleural effusions compared to DRibbles from the NSCLC cell line UbiLT3. Autophagosomeenriched DRibbles for patients 2, 3, 5, 6, and UbiLT3 DRibbles, derived from a lung tumor cell line, used as a control, were labeled with autophagosome specific antibodies [anti-LC3 (PE), anti-LC3 (PE)/LAMP1-FITC, anti-p62 (PE), or anti-p62 (PE)/LAMP1-FITC]. Normal rabbit IgG (isotype control; i.c.) and CD3-PE were used as controls. Patient-derived DRibble preparations were more heterogeneous compared with cell-line derived UbiLT3