Fig. 2.

HRCHs modulated the expression of proteins on D-384 cells. D-384 cells were exposed to IC₅₀ of HRCH 1, HRCH 7, and HRCH 9 for 48 h. a Total protein lysates were separated by 12–15% SDS-PAGE followed by western blot analysis with the indicated antibodies against p-73, pY99-TP73, pS46-TP53, Bcl-2, Bax, p21, γH2A.X, pS139-H2A.X and β-tubulin. b Quantitative analysis with software C-Digit Blot scanner. β-Tubulin was used as a loading control for total cell extracts