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. 2017 Dec 18;12:81. doi: 10.1186/s40793-017-0293-8

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Synteny comparison of Hpr-2 type-3 secretion systems (T3SS) used in the phylogenetic tree. Evolutionary distance was computed with MEGA7 using a maximum-likelihood method. The bootstrap values above 50% (from 1000 replicates) are indicated at the nodes. The tree was generated based on alignment of eight conserved genes of the T3SS (SctS, SctR, SctQ, SctV, SctU, SctJ, SctN, and SctT) indicated by the colored boxes. The letter in the boxes indicates the last letter of the Sct gene. Comparison percentage was generated using BLAST+ 2.4.0 (tBLASTx with cutoff value 10⁻³) and map comparison figures were created with the Easyfig program [33]