Table 3.
Agrobacterium-mediated transformation of switchgrass Alamo calluses without pre-selection of calluses types
| Vector | Experiment | No. of calluses inoculated | No. of hygromycin resistant callus after 6 weeks selection | No. of regenerated callus | No. of rooting seedling | No. of plants positive for genomic DNA PCR | Selection frequency (%) |
|---|---|---|---|---|---|---|---|
| pCAMBIA-EV | 1 | 26 | 19 | 4 | 4 | 4 | |
| 2 | 31 | 22 | 4 | 4 | 4 | ||
| 3 | 33 | 19 | 5 | 4 | 4 | ||
| Total | 90 | 60 | 13 | 12 | 12 | 100.0 | |
| pCAMBIA-RFP | 1 | 26 | 18 | 4 | 5 | 2 | |
| 2 | 34 | 23 | 6 | 5 | 5 | ||
| 3 | 35 | 22 | 7 | 4 | 4 | ||
| Total | 95 | 63 | 17 | 14 | 11 | 78.6 | |
| pCAMBIA1305.2 | 1 | 20 | 19 | 5 | 4 | 1 | |
| 2 | 30 | 23 | 5 | 4 | 3 | ||
| Total | 50 | 42 | 10 | 8 | 4 | 50.0 |
Selection efficiency: number of genomic DNA PCR positive switchgrass transformants divided by the regenerated and rooted hygromycin B resistant switchgrass seedlings