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. Author manuscript; available in PMC: 2017 Dec 19.
Published in final edited form as: Sci Signal. 2015 Jun 16;8(381):ra59. doi: 10.1126/scisignal.aaa2155

Fig. 5. Positive autoregulation of IL-6 may increase cell-to-cell heterogeneity when single cells are isolated.

Fig. 5

(A) Distribution of the amounts of IL-6 secreted above the BT by 202 cells after 20 hours of incubation with LPS alone (red) or in the presence of TNF (gray). (B) Normalized concentrations of IL-6 secreted by a cell population after 20 hours of treatment with LPS alone, IL-6 alone (100 pg/ml), LPS with an anti-IL-6R antibody, or LPS with recombinant IL-6. The amounts of IL-6 secreted were measured by ELISA, and the final IL-6 concentration was calculated as [concentration measured in sample – concentration measured for recombinant IL-6 without cells]. Data are normalized to treatment with LPS alone and are means ± SEM of two or three biological replicates. *P < 0.05 by t test.