Survival experiments. A, Experimental setup and treatment schedule is depicted. Anti-PD-1 antibody was administered on days 10, 12, and 14. Anti-TIM-3 antibody was given on days 7, 11, and 15. 10 Gy of radiation was delivered by a SARRP instrument. SARRP, Small Animal Radiation Research Platform. B, Kaplan–Meier curve. P < 0.05 between triple therapy group (anti-PD-1 + anti-TIM-3 + SRS) and all other arms. Survival experiment was repeated in triplicate and a representative repeat was presented, n =10. Survival was analyzed by Kaplan–Meier method and compared by log-rank Mantel–Cox test. *, P < 0.05.