Fig. 7.

Transplantation of hAD-MSCs reduces ovarian CG apoptosis in POI rats. a Dark brown cells indicate the ovarian apoptotic cells stained by TUNEL apoptosis assay kit (40× and 200×). b,c Bax and Bcl-2 expression levels were determined by Western blot analysis (b), and the Bcl-2/Bax ratios were evaluated (c). Scale bars = 100 μm. *P < 0.05, **P < 0.01; ^## P < 0.01. hAD-MSCs human adipose-derived mesenchymal stem cells, LIPUS low-intensity pulsed ultrasound, POI primary ovarian insufficiency