Table 1.

Sequences of primers and real-time polymerase chain reaction conditions

Gene		Primer sequences
Cbfα1	Forward	5′-GTCTTACCCCTCCTACCTGA-3′
	Reverse	5′-TGCCTGGCTCTTCTTACTGA-3′
ALP	Forward	5′-ACGTGGCTAAGAATGTCATC-3′
	Reverse	5′-CTGGTAGGCGATGTCCTTA-3′
COL I	Forward	5′-TGTTCAGCTTTGTGGACCTC-3′
	Reverse	5′-CTTGGTCTCGTCACAGATCA-3′
OCN	Forward	5′-CAAAGGTGCAGCCTTTGTGTC-3′
	Reverse	5′-TCACAGTCCGGATTGAGCTCA-3′
β-actin	Forward	5′-ATCATGTTTGAGACCTTCAA-3′
	Reverse	5′-CATCTCTTGCTCGAAGTCCA-3′

ALP alkaline phosphatase, Cbfα1 core-binding factor α1, COL I collagen-1, OCN osteocalcin