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. 2017 Dec 19;14:253. doi: 10.1186/s12974-017-1024-1

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — The LPAR5/PKD axis controls the phosphorylation of pro-inflammatory transcription factors. a PMM were seeded on 12-well plates, serum-starved, and incubated with DMSO, DMSO plus LPA (1 μM), and LPA (1 μM) in the presence of TCLPA5 (5 μM) or CRT0066101 (1 μM) for the indicated time periods. The phosphorylation of p65-NF-κB, STAT1, STAT3, and c-Jun was detected by western blotting. One representative blot is shown (N = 3). b Densitometric analysis of western blots (N = 3). Results are presented as mean values + SEM (***p < 0.001, compared to DMSO-treated cells; ^## p < 0.01, ^### p < 0.001, LPA plus TCLPA5 or CRT versus DMSO + LPA; one-way ANOVA with the Bonferroni correction)