Table 3.

Microarray validation by qPCR for the HT-E₂ vs. HT comparison

Microarray			qPCR
Gene symbol	log2 Fold change	Adjusted P -value	log2 Fold change	Adjusted P-value
amh	−2.48	0.037*	−3.18	0.011*
aqp1	2.16	0.349	0.70	0.094
col18a	−4.28	0.035*	−0.78	0.174
cyp19a1a	−1.03	0.781	−3.47	0.000***
cyp19a1b	−1.37	0.089	0.12	0.028*
dmrt1	−1.54	0.007**	−2.39	0.004**
gnrh	1.11	0.129	−1.83	0.051
igf1	5.95	0.035*	−1.73	0.149
mettl22	−1.31	0.121	−1.21	0.113
prl	−1.03	0.806	−1.94	0.018*
sox17	1.08	0.779	0.35	0.570
star	−1.87	0.051	−2.00	0.006**
tesc	−1.17	0.324	−1.88	0.017*
vasa	–	–	−0.64	0.721
wisp1	1.02	0.816	−1.51	0.045*

Note: Asterisks note statistical significant differences: * = P < 0.05; ** = P < 0.01; *** = P < 0.001. N 10 individuals analyzed per gene