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. Author manuscript; available in PMC: 2017 Dec 19.
Published in final edited form as: Cell Rep. 2017 Dec 5;21(10):2978–2991. doi: 10.1016/j.celrep.2017.11.041

Figure 7. A systematically inducible activation of different classes of pharyngeal neurons.

Figure 7

(A) Mean preference index values from binary choice experiments with capsaicin tested against ethanol solvent. Genetic manipulations were performed in poxn mutant (poxnΔM22-B5/poxn70). Two different UAS-VR1E600K controls are shown; [1] is a recombinant of UAS-VR1E600K with the poxnΔM22-B5 allele; [2] is a recombinant with the poxn70 allele. n=19–30. Error bars indicate SEM. ¶ indicates significant difference from the corresponding UAS control; ξ indicates significant difference from the corresponding GAL4 control (for double driver experiments, ξ indicates significant difference from both GAL4 controls); P<0.05, one-way ANOVA with Tukey test. ns, not significant.

(B) Mean preference index values from binary choice experiments with capsaicin-sucrose mixture tested against sucrose alone. Genetic manipulations were performed in poxn mutant (poxnΔM22-B5/poxn70). n=28–32. Error bars indicate SEM. ¶ indicates significant difference from the corresponding UAS control; P<0.05, one-way ANOVA with Tukey test.

(C) Results of binary choice feeding assays performed using flies expressing VR1E600K under the control of the indicated GAL4 drivers in a wild type background with only one poxnΔM22-B5 allele. Tastants used were capsaicin and ethanol solvent. n=10–20. Error bars = SEM. ¶ indicates significant difference from the UAS control; ξ indicates significant difference from the corresponding GAL4 control; P<0.05, one-way ANOVA with Tukey test. ns, not significant.