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. Author manuscript; available in PMC: 2017 Dec 19.
Published in final edited form as: Aquaculture. 2006 May 20;259(1-4):403–414. doi: 10.1016/j.aquaculture.2006.05.011

Fig. 2.

Fig. 2

Motility (mean±SD) of sperm from X. helleri when suspended in Hanks’ balanced salt solution (HBSS) at 300 mOsm/kg and subjected to different treatments: without centrifugation (open circles), centrifuged once without removal of supernatant (filled circles), centrifuged once with removal of supernatant and subsequent replacement of fresh HBSS (open triangles), and centrifuged twice with removal of supernatant and subsequent replacement of fresh HBSS (filled triangles). Samples were centrifuged at the speed of 600 ×g (upper panel), 1000 ×g (middle panel), and 1200 ×g (lower panel). Motility was monitored at 2, 16, 48, 96, 168, and 240 h after treatment.