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. Author manuscript; available in PMC: 2017 Dec 19.
Published in final edited form as: Aquaculture. 2006 May 20;259(1-4):403–414. doi: 10.1016/j.aquaculture.2006.05.011

Fig. 3.

Fig. 3

Motility (mean±SD) of sperm from X. helleri when suspended in Hanks’ balanced salt solution (HBSS) at 300 mOsm/kg and equilibrated without glycerol (open circles) and with 14% glycerol for 1 h (open triangles), 2 h (open diamonds), 5 h (filled circles), 24 h (filled triangles). After treatment, samples were centrifuged at 1000 ×g for 10 min at 4 °C, and supernatant were removed and replacement of fresh HBSS. Motility was monitored immediately after centrifugation and 48, 120, and 192 h after treatment.