Motility (mean±SD) of sperm from X. helleri when suspended in Hanks’ balanced salt solution (HBSS) at 300 mOsm/kg, centrifuged at 1000 ×g for 10 min at 4 °C with the removal of supernatant, and replaced with HBSS (filled circles), HBSS without potassium (open circles), calcium (filled triangles), magnesium (open triangles), and glucose (open squares), and 0.9% saline solution (filled squares). This process was repeated three times, and motility was monitored at 2, 14, 36, 60, 104, 128, 168, and 216 h after the final centrifugation.