Motility (mean±SD) after thawing of X. helleri sperm cryopreserved with combined glycerol and glucose at different concentrations: 14% glycerol and 1 g/L glucose (open triangles), 10% and 25 g/L (open circles), 12% and 25g/L (filled circles), and 14% and25g/L (filled triangles), and centrifuged at 1000 ×g for 10 min at 4 °C with the removal of supernatant, and replacement of fresh HBSS. Motility was monitored at 2, 18, 42, 66, 90, 138, and 182 h after centrifugation.