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The effects of tetramethylpyrazine (TMP) on HepG2 cell apoptosis using high-content screening (HCS) analysis. (A) HepG2 cells were stained by Hoechst 33342 and Annexin V-FITC/PI (propidium iodide) fluorescent dyes, and (B) the nucleus size, (C) Annexin V-FITC fluorescence, and (D) PI fluorescence were evaluated by HCS. Data are presented as mean ± SD from triplicate samples. *P < .05, **P < .01 compared with the control group.
