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. 2016 May 13;15(2):226–236. doi: 10.1177/1534735416637424

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Figure 4. — The mitochondrial function induced by tetramethylpyrazine (TMP) in apoptotic HepG2 cells. Cells were treated with TMP for 48 hours at a concentration of 700 µmol/L and then, respectively stained with the Hoechst 33342, cytochrome c, and mitochondrial membrane potential (Δψm). The release of cytochrome c from mitochondria to cytosol is shown in image (A) and quantification of the average cytochrome c intensity in (B). The cell Δψm is shown in image (C) and quantification of the average Δψm intensity in (D). Data are presented as mean ± SD from triplicate samples. *P < .05 and **P < .01 compared with the control group.