Figure 5.

The activation of caspases induced by tetramethylpyrazine (TMP) in apoptotic HepG2 cells. HepG2 cells were incubated with TMP for 48 hours at a concentration of 700 µmol/L, and then, cleaved-caspase-3, cleaved-caspase-9, and β-actin were assessed via simple Western blotting. (A-C) The electropherogram, (D) pseudo-gel, and (E) quantification of simple Western blotting were measured using antibody for blotting. Data are presented as mean ± SD from triplicate samples. *P < .05, **P < .01 compared with the control group.