Figure 2.

Eupolyphaga sinensis Walker 70% ethanol extract (ESWE) suppressed ERK1/2 activity in breast cancer cells. MDA-MB-435s (A) and MDA-MB-231 (B) cells were incubated with ESWE at different concentrations for 48 hours. Whole-cell extracts were prepared and analyzed by western blot analysis with antibodies against p-ERK1/2, ERK1/2, and GAPDH (glyceraldehyde 3-phosphate dehydrogenase). C. Quantification of (A) and (B). The results shown were representative of 3 independent experiments.^a

^a*P < .05, **P < .01 compared with untreated control cells.