Figure 3.

Eupolyphaga sinensis Walker 70% ethanol extract (ESWE) downregulated CXCR4 in breast cancer cells: MDA-MB-435s (A) and MDA-MB-231 (B) cells were incubated with ESWE at different concentrations for 48 hours. Whole-cell extracts were prepared and analyzed by western blot analysis with antibodies against CXCR4. The same blots were stripped and reprobed with GAPDH antibody to show equal protein loading. C. Quantification of (A) and (B). The results shown are representative of 3 independent experiments.^a

^a*P < .05, **P < .01 compared with untreated control cells.