Figure 5.

Eupolyphaga sinensis Walker 70% ethanol extract (ESWE) suppressed CXCR4 partial by proteasomal degradation: MDA-MB-435s (A) and MDA-MB-231 (B) cells were treated with indicated concentrations of chloroquine or MG132 for 1 hour, followed by treatment with ESWE for 48 hours. Whole-cell extracts were prepared and analyzed by western blot analysis using antibodies against CXCR4. The same blots were stripped and reprobed with GAPDH antibody to show equal protein loading. C. Quantification of (A) and (B). The results shown are representative of 3 independent experiments.