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Combinations of primers used for PCR amplification
| Target gene | Forward primer | Reverse primer | T (°C)‡ |
|---|---|---|---|
| cox1 | OomCox1-levup | OomCox1-levlo | 50, 55 |
| FM85mod | 50, 55 | ||
| cox2 | Cox2-F | Cox2-R | 50, 55 |
| Cox2-RC4 | 50 | ||
| cox2-1 spacer† | FM79-C1 | FM80 | 52 |
| FMPhy-10b | 52 | ||
| OomCoxS-R2 | 52 | ||
| cox2+ cox2-1 spacer | Cox2-F | FMPhy-10b | 54 |
| OomCoxS-R3 | 54 |
The spacer region between cox2 and cox1.
Annealing temperature during amplification.
