Fig 4. Variation of ΔT_m at increasing ratios of cdh23 and pcdh15.

(A) Variation of ΔT_m for WT and two deafness-related complexes at increasing concentration ratios. The WT ΔT_m increases from ~2 to ~5.5°C from 1:1 to 5:1 ratios. The deafness mutants have ΔT_m < ~1°C at all ratios. (B) ΔT_m variation of the most stabilizing mutant G16D-T15E (green), the WT complex (blue), and the less stabilizing mutant Q165L (pink). The double mutant has higher ΔT_m than the WT complex at all ratios. (C) Bar graph of ΔT_m variation of selected mutants at different cdh23:pcdh15 ratios. Asterisks indicate statistically significant differences with WT complex (p ≤ 0.05). Data for all mutants including pcdh15- A20V, D160W, L187W, Q165L, S92V and cdh23-T190W are shown in Table 1.