Fig 7. The CD8⁺ T cell population in S1L infected TG contract more rapidly and contain a higher frequency of active non-gB-CD8s.

B6 mice received corneal infections with either WT or the S1L mutant at 1x10⁵ PFU/cornea. TGs were harvested at 8, 12, 16, 20, or 30 dpi and: (A) stained for CD45, CD3, and CD8, analyzed by flow cytometry, and data recorded as the mean number of CD8⁺ T cells/TG; or stimulated for 6 hrs with (B) HSV-1 gB-null-EGFP infected or (C) PRV-gB infected B6WT3 fibroblasts in the presence of Brefeldin A. The cells were then stained for surface CD45, CD3, and CD8 and for intracellular IFNγ. Data in B and C are presented as the mean ± SEM frequency of IFNγ⁺ CD8⁺ T cells in each TG as a fraction of total CD8⁺ T cells. * p<0.05, ** p<0.01, ***p<0.001 based on a t-test comparison at each time.