| Antibodies |
| Anti-HA |
ABM |
G166 |
| Anti-HA (12AC5) |
Liz Miller (gift) |
- |
| GAPDH |
Sigma |
G9545 |
| MPK1 |
Santa Cruz |
Sc-374434 |
| HOG1 |
Santa Cruz |
Sc-165978 |
| Anti-Rpb3 |
Biolegend |
665004 |
| Anti-Y1P (3D12) |
Active Motif |
61384 |
| Anti-Ser2P (3E10) |
Dirk Eick (gift) |
- |
| Anti-Ser5P (3E8) |
Millipore |
04-1572 |
| Anti-Rpb1 (y80) |
Santa Cruz |
Sc-25758 |
| Anti-Rpb1 (8WG16) |
Santa Cruz |
Sc-56767 |
| Anti-Ser5P (H5) |
Biolegend |
920204 |
| Chemicals, Peptides, and Recombinant Proteins |
| Nourseothricin (NAT) |
Werner Bioagents |
5.001.000 |
| G418 (Geneticin) |
Invivogen |
Ant-gn-5 |
| Random Hexamer Primer |
Thermo Scientific |
SO142 |
| GST-CTD |
Hirose and Manley 1998 |
- |
| Phleomycin |
Invivogen |
Ant-ph-1 |
| Calcofluor White |
Sigma-Aldrich |
18909 |
| FK506 |
Invivogen |
Tlrl-fk5 |
| Deposited Data |
| SGA Dataset (Table S2) |
This paper |
doi:10.17632/8fvx64vvpg.1 |
| 3′READS Dataset (Table S3) |
This paper |
doi:10.17632/8fvx64vvpg.1 |
| Experimental Models: Organisms/Strains |
| Saccharomyces cerevisiae background BY4741 |
ATCC |
ATCC: 4040002 |
| Saccharomyces cerevisiae background Y7092 |
Boone Lab |
- |
|
Please see
Table S1
for all derivative strains
|
- |
- |
| Software and Algorithms |
| Bowtie2 |
Langmead and Salzberg, 2012 |
http://bowtie-bio.sourceforge.net/bowtie2/index.shtml |
| DESeq |
Love et al. 2014 |
- |
