Table 3.
Primers.
| Primers | Sequence (5’-3’) | Description |
|---|---|---|
| sucA-1 | AAGCATAAAAAAGATGCTTAAGGGATCACGTAACGGCTGACATGGGAATTAG* | Deletion of sucA |
| sucA-2 | TATCTACGCTACTCATTGTGTATCCTTTATTTGAGCGATTGTGTAGGCTGG | |
| sucA-3 | ACGTAGACAAGAGCTCGCAAGTG | Identification for sucA deletion |
| sucA-4 | CGGGTTTTTTATGCCAGGTTG | |
| aceA-1 | AACCACCACATAACTATGGAGCATCTGCACTAACGGCTGACATGGGAATTAG | Deletion of aceA |
| aceA-2 | CGGCCTACAGTCAGCAACGGTTGTTGTTGCTTGAGCGATTGTGTAGGCTGG | |
| aceA-3 | TGGAACAGATCACCACTTCCG | Identification for aceA deletion |
| aceA-4 | ATGATAAGACGCGCAAGCGT | |
| ido-1 | GCCCGGGGGATCCACTAGTTAAGGAAGCTAGATATGAAAATGAGTGGCTTTAGCATAG | Amplification of ido and expressed by pWSK29 |
| ido-2 | GGTGGCGGCCGCTCTAGTTATTTTGTCTCCTTATAAGAAAATGTTACTA | |
| ido-3 | GGATCCATGAAAATGAGTGGCTTTAGCATAG(BamH I) | Amplification of ido and expressed by pET-His |
| ido-4 | AAGCTTTTATTTTGTCTCCTTATAAGAAAATGTTACTA(Hind III) |
*
Underlined letters indicate the sequence 30 bp upstream or downstream of the gene (or 20 bp upstream or downstream of Xba I recognition site of pWSK29) to be deleted are underlined. Recognition sites are in italics and restriction enzymes are shown in parentheses.