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. Author manuscript; available in PMC: 2017 Dec 19.
Published in final edited form as: Biomacromolecules. 2017 Oct 11;18(11):3753–3765. doi: 10.1021/acs.biomac.7b01349

Figure 3.

Figure 3

NP-mediated delivery of siRNA results in robust gene silencing across multiple cell types and species. Murine tenocytes (A), murine MSCs (mMSC) (B), and human MSCs (hMSC) (C) were treated with 30 nM siRNA targeting glyceraldehyde 3-phosphate dehydrogenase (GAPDH) complexed to NP at a charge ratio of 4:1. Gene expression was detected using RT-PCR and normalized to negative controls in each cell type. GAPDH expression was normalized to B-Actin expression for murine cell types and peptidylprolyl isomerase B (PPIB) expression for hMSCs. *p < 0.0001 compared to negative control, #p < 0.001 compared to positive controls (+) control. Negative controls (–) are comprised of untreated cells and positive controls (+) are cells treated with 30 nM anti-GAPDH siRNA using Lipofectamine2000.