Exposure to 30 minutes of six different types of dung biomass smoke does not cause cytotoxicity in SAECs.
SAECs were exposed to air or dung smoke (horse, U.S. cow, India cow, elephant, goat, or rhinoceros (rhino)) for 30 minutes. Alamar Blue reagent was added to the cells 24-hours post-exposure. After a 4-hour incubation, cell supernatants containing Alamar Blue were collected. Reduction of Alamar Blue was determined by measuring the fluorescence of the samples (excitation = 560 nm and emission = 590 nm). Data represent mean (n = 3 replicates per exposure group from an independent experiment).