Cox-2 protein expression is upregulated by six different types of dung biomass smoke.
SAECs were exposed to air or dung smoke (horse, U.S. cow, India cow, elephant, goat, or rhinoceros (rhino)) for 30 minutes and cell lysates were collected 24-hours post-exposure. (A) Representative Western blot showing 3 replicates per condition of Cox-2 protein expression and Ponceau S staining in SAECs exposed to air or different types of dung smoke. (B) Densitometry of Cox-2 protein levels was performed. Data represent mean ± SD (n = 3 replicates per exposure group from 1 of 3 independent experiments), *p<0.05 by one-way ANOVA (compared to air-exposed cells using Tukey’s post-hoc analysis).