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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: J Immunol. 2017 Nov 29;200(1):110–118. doi: 10.4049/jimmunol.1701133

Figure 5.

Figure 5

STAT5B deficiency results in cell death resistance of CD4+TEM cells upon TCR restimulation in mice and humans.

(a) Naïve and TEM T cells were isolated from WT (blue) or Stat5b−/− (red) mice and then stimulated in vitro for 24 hours with 100U/mL of rhIL-2 and increasing concentration of plate-bound anti-CD3 (from left to right: 0.1, 1 and 10 μg/mL). Cell loss was assessed as in (Fig. 1b). (b) TCM and TEM cells were isolated from peripheral blood from normal control subject (blue) and patient (red), then stimulated in vitro for 24 hours with plate-bound anti-CD3 at different concentrations (from left to right: 0.1, 1 and 10 μg/mL) and cell loss was calculated as in (a). *** p=<0.001. Data are representative of at least two (a) and three (b) independent experiments.