Skip to main content
. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Int J Radiat Oncol Biol Phys. 2017 Sep 21;100(1):162–173. doi: 10.1016/j.ijrobp.2017.08.033

Fig. 1.

Fig. 1

siRNA knockdown of ATIC is radiosensitizing. (A) GM00637 cells were transfected with pooled siRNAs targeting ATM (siATM), NBN (siNBS1), MTPAP (siMTPAP) or ATIC (siATIC). The mean percentage ± SEM of viable cells in the irradiated (4Gy) samples relative to mock-irradiated is plotted (*P < 0.05, **P<0.01, compared to siControl). (B) The fraction of transcripts in cells treated with gene-specific siRNAs compared to siControl is plotted. (C) Cells treated with either siControl or the pools of siRNAs directed against the indicated genes were immunoblotted for the products of the targeted genes. (D) Protein levels from (C) quantified and expressed as percentage relative to siControl.