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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Int J Radiat Oncol Biol Phys. 2017 Sep 21;100(1):162–173. doi: 10.1016/j.ijrobp.2017.08.033

Fig. 4.

Fig. 4

ATIC inhibition or depletion increases the incidence of DNA double-strand breaks in HCT116 colon cancer cells after irradiation. (A) γH2AX staining at 0 hour and 1 hour after irradiation (2 Gy). Cells were sham treated, treated with siATICa or with Cpd14 at the indicated concentration for 48 hours prior to radiation exposure (IR). (B) Percentage γH2AX positive cells in treated and control groups. * indicates P<0.05. (C) Neutral comet assay performed at 0 hour and 1 hour after irradiation (2 Gy). Cells were sham treated, treated with siATIC or with Cpd14 at the indicated concentration for 48 hours prior to radiation exposure. (D) Quantitation of tail moments in treated and control groups (*P<0.05).