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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Biochem Biophys Res Commun. 2017 Nov 9;495(1):433–437. doi: 10.1016/j.bbrc.2017.11.058

Figure 2. Cell-free hemoglobin increases endothelial macromolecular permeability without altering viability.

Figure 2

HUVECs were cultured to confluence on transwell plates and treated with cell-free hemoglobin (CFH, 0–1 mg/ml) for 18 h. CFH increased transfer of 14C-inulin (A) in a dose-dependent manner. Cell viability as measured by Trypan blue exclusion was not affected by CFH treatment for 18 hours (B). *p < 0.05 by ANOVA.