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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Biochem Biophys Res Commun. 2017 Nov 9;495(1):433–437. doi: 10.1016/j.bbrc.2017.11.058

Figure 4. Ascorbate prevents increases in endothelial permeability caused by CFH.

Figure 4

HUVECs were cultured to confluence in antioxidant-free medium on transwell plates and loaded with ascorbate (0–60 uM) for 15 min prior to challenge with cell-free hemoglobin (CFH, 0.3 mg/ml) for 18 h. Ascorbate significantly blocked CFH mediated decrease in electrical resistance (A) and the increased transfer of 14C-inulin (B). *p < 0.05 by ANOVA.