Fig.1.

Knockdown of endogenous Bit1 expression in BEAS-2B cells promotes growth and anoikis resistance. A. Stable control shRNA and Bit1 shRNA pool of BEAS-2B cells were subjected to total cell lysate isolation, SDS-PAGE, and immunoblotting against specific antibodies to Bit1 and B-actin. B. The growth of control shRNA and Bit1 shRNA cells was quantified by examining the number of metabolically active cells at the indicated time points with the use of alamar blue assay as described in materials and methods. C. and D. The growth of control shRNA and Bit1 shRNA cells was also examined by clonogenic assay. The representative colonies were visualized by methylene blue staining (C) and quantified (D). E. Control shRNA and Bit1 shRNA cells were cultured in attached and detached conditions for 24h and then harvested for Cell Death Elisa as described in materials and methods. In B, D, and E, three independent experiments were performed in triplicates, * indicates p<0.05 by Student's t test.