Skip to main content
. Author manuscript; available in PMC: 2018 Dec 19.
Published in final edited form as: Anal Chem. 2017 Nov 28;89(24):13227–13234. doi: 10.1021/acs.analchem.7b03070

Figure 5. Detection of in vitro protein synthesis in the ICEcreamer incubator.

Figure 5

The eGFP-encoding DNA construct contains the eGFP open reading frame, a T7 RNA polymerase promoter (T7) to initiate transcription, an epsilon enhancer (ε), and a ribosome binding site (RBS). DNA templates encoding eGFP were transcribed and translated at ambient temperature with incubation in the 2×-wide 2×-long ICEcreamer (26-min incubation). Streptomycin inhibited eGFP expression in positive control droplets (Z′ = 0.64). Each histogram represents 5,000 droplets.