Figure 3.

TET2 loss enhances memory CD8⁺ T cell differentiation. Control and TET2cKO mice were infected with LCMV and gp33⁺ CD8⁺ T cells isolated from spleen and lymph nodes were evaluated at indicated time-points. A) Frequency (left) and absolute number (right) of SLEC and MPEC populations among gp33⁺ CD8⁺ TCRβ⁺ splenocytes from control and TET2cKO mice isolated D8 p.i. B) Left, representative flow cytometric analysis of CD62L expression on gp33⁺CD8⁺ T cells. Right, frequency and absolute number of CD62L⁻ effector memory (EM) and CD62L⁺ central memory (CM) populations among gp33⁺ CD8⁺ TCRβ⁺ splenocytes from control and TET2cKO mice isolated D45 p.i. C) Representative flow cytometric analysis of CD27 (top) and CXCR3 (bottom) expression on gp33⁺CD8⁺ T cells on day 45 p.i.. D) Absolute number of gp33⁺ CD8⁺ TCRβ⁺ lymphocytes isolated from lymph nodes of control and TET2cKO mice on D45 p.i. E) Representative flow cytometric histograms of intracellular Eomes expression in gp33⁺ CD8⁺ TCRβ⁺ splenocytes isolated from control and TET2cKO mice on D45 p.i. Plots are gated on live, gp33⁺CD8⁺TCRβ⁺ lymphocytes. Data representative of n=9/control and 12/TET2cKO, 3 independent experiments (A), n=10/genotype, 2 independent experiments (B–E). *p<0.05, ***p<0.001, unpaired t-test.