Table 2.
In vitro anti-proliferative activities of N1-monosubstituted and N1,N3-disubstituted thiobarbituric acid derivatives on melanoma CHL-1 and UACC 903 cancer cells (72 h treatment).a
| Compounds | Melanoma cells IC50 (μM)
|
|
|---|---|---|
| CHL-1 | UACC 903 | |
| 8a | 7.5 ± 1.7 | 6.7 ± 1.2 |
| 8b | 3.8 ± 1.3 | 3.8 ± 0.4 |
| 8c | 4.7 ± 0.5 | 5.5 ± 1.9 |
| 8d | 13.9 ± 2.3 | 16.2 ± 3.0 |
| 8e | 4.7 ± 1.1 | 4.6 ± 0.9 |
| 8f | 6.2 ± 2.2 | 8.0 ± 1.8 |
| 8g | 23.4 ± 1.7 | 23.4 ± 3.6 |
| 8h | 7.5 ± 2.8 | 7.6 ± 3.9 |
| 8i | 8.6 ± 1.9 | 18.8 ± 2.7 |
| 8j | 8.8 ± 3.1 | 15.2 ± 2.3 |
| 8k | 18.9 ± 2.8 | 14.5 ± 1.7 |
| 8l | 20.4 ± 3.7 | 21.0 ± 3.6 |
| 8m | 6.1 ± 2.1 | 6.1 ± 1.4 |
| 8n | 8.0 ± 2.9 | 17.6 ± 2.9 |
| 8o | 8.1 ± 0.7 | 18.1 ± 3.2 |
| PLX-4032 | 13.7 ± 1.7 | 3.6 ± 1.1 |
a
Values are the mean of triplicates of at least two independent experiments. Non-linear graphs using Prism software were used to determine Mean ± SD values.