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. 2017 Dec 19;7:17785. doi: 10.1038/s41598-017-18138-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of miR-196a and miR-196b target genes in HNSCC cells and CAFs. mRNA expression levels of potential/validated miR-196a/b target genes were measured by RT-qPCR in (A) UT-SCC-42B, (B) FaDu and (C) CAFs after 72 h transfection with either premiR-196a, premiR-196b or non-targeting control. Data were normalized to RPL19 levels and relative to control-transfected cells. The graphs represent the mean ± SD, calculated from at least three independent experiments performed in triplicate. ND, not detected. *p < 0.05, **p < 0.01 and ***p < 0.001 by Student t-test.